Expert Pathology and Histology: Foundation for Preclinical Advancement 

Comprehensive Histopathological and Specialized Services

At Veranex, we offer a wide range of histopathological techniques and specialized services to meet the diverse needs of our clients in research, preclinical, and clinical fields. Our services are designed to provide high-quality, reliable results that support the safety, performance, and efficacy assessments of new biomaterials and devices.

• Histopathological Techniques

  • Tissue Trimming: Trimming of tissues and organs.
  • Decalcification: Specific protocols for effective decalcification of tissues.
  • Frozen Sections: Provision of frozen sections using a cryostat and experienced staff.
  • Custom Staining: Protocols, dyes, and solutions for routine and special staining techniques.
  • IHC and IF Staining: Optimization and validation of immunohistochemical and immunofluorescent staining.
  • Traveling Necropsy: Off-site prosection services for medical devices, biopharmaceuticals, and biologic therapies.
  • Paraffin Embedding & Sectioning: Standard paraffin processing, embedding, and microtomy.
  • Histochemical Staining: A wide range of routine and special histochemical stains.

• Specialized Services

  • Immunohistochemistry: Development and application of routine and novel immunohistochemical markers.
  • Slide Imaging/Scanning: High magnification slide scanner for online viewing and file delivery.
  • Necropsy Services: Supervised necropsies and macroscopic evaluations by certified veterinary pathologists.
  • General Histology Services: Routine histology including paraffin embedding, sectioning, and special stains.

• Specialized Tissue Processing

  • PMMA and Spurr Resin Embedding: For evaluation of large or hard implants in undecalcified tissues.
  • EXAKT® Cutting and Grinding: Techniques for precise tissue analysis.
  • Whole Slide Imaging: Digitalization of histological slides for archiving and telepathology.

• Histopathological Evaluation

  • Morphometry: Quantitative measurement of histological parameters using professional software.
  • Pathology Reporting: Comprehensive, illustrated reports including narrative descriptions and quantitative assessments.

• Additional Offerings

  • Microphotographing: Remarkable microphotographs for regulatory purposes, illustration of histological observations and publication.
  • Customized Measurements: Tailored evaluations to meet specific study needs.
  • Oral Presentations: Scientific discussions and presentations with study pathologists upon request.

• General Histology Services

  We offer the following routine histology services:

  • Paraffin embedding and sectioning
  • Routine HE&S staining
  • Frozen Sectioning
  • Special Stains

   

  Applications

  • Cusps from prosthetic valves
  • Soft devices or biomaterials (vascular conduits, artificial ureters, bladder, surgical sealants, patches, etc.)
  • Target/distant organs (kidney, liver, lung, or brain) for the assessment of systemic effects

  Special stains are very useful in the qualitative assessment of histological sections and complement HE&S as they specifically stain some components of normal or pathological tissues. We developed routine panels of special stains for cusps of prosthetic valves, cartilage, biomaterials, or other tissues.

• Immunohistochemistry

  • Specific immunolabelling is a valuable ancillary method to visualize and quantify cells or tissue components. We perform immunohistochemistry for endothelial cells, collagen fibers, smooth muscle cells, myofibroblasts, or inflammatory cell populations and sub-populations, and can also develop personalized protocols to fit your needs and endpoints.

• Specialized Tissue-Implants Processing

  • PMMA and Spurr Resin Embedding
  • EXAKT® Cutting and Grinding
  • H&E and Masson Trichrome Staining of Resin Sections

  This highly-specialized technique is typically used for the evaluation of large and/or hard implants or soft/hard implants in undecalcified tissues. It allows maintaining tissue-implant integrity, enabling accurate evaluation of the intimate interface between the biomaterial and implanted tissues.

   

  Applications

  • Heart valves
  • Metallic stents
  • Orthopedic implants
  • Artificial cartilage
  • Ophthalmic implants
  • etc.

• Whole Slide Imaging

  • We can take over the digitalization of histological slides for permanent archiving, telepathology, or quantitative measurements.

• Histopathological Evaluation

  The microscopic evaluation of your study will be performed by certified, experienced veterinary pathologists. The study pathologist will qualitatively describe and interpret the microscopic findings with regard to the study endpoints, using international histological guidelines. A semi-quantitative score of severity with predetermined criteria will be applied. These criteria are defined to meet the specific aims of your study. The pathologist will also take remarkable microphotographs of representative findings from the study that are suitable for regulatory purposes and for publishing.

  Histopathological evaluations are conducted according to internal standard operating procedures and to current highest professional and scientific standards in the spirit of ISO10993-Part 6: Tests for Local Effects after Implantation and ISO10993-Part 11: Test for Systemic toxicity. All evaluations can be conducted in compliance with Good Laboratory Practice (GLP) and audited by the Quality Insurance Unit of Veranex.

• Morphometry

  Quantitative parameters (fibrous pannus thickness, remnants of the implanted biodegradable material, bone-implant contact, or vessel stenosis, etc.) can be measured on histological sections with professional software. We use dedicated protocols for the histomorphometric evaluation of heart valves, stents, bone reconstruction, and dental implants. Customized measurements can also be applied to fit your specific needs.

• Pathology Reporting

  Our hallmark is clear, didactic, and richly illustrated reports with annotated pictures.

  The histopathology report includes:

  • Narrative description of microscopic changes and interpretation
  • Qualitative and semi-quantitative assessment of microscopic changes
  • Individual data
  • Representative microphotographs Histomorphometry data

   

  In addition:

  • The images can be professionally edited for publication-ready submission
  • An oral presentation of the pathological data and scientific discussion with the study pathologist and other members of the scientific staff can be organized upon request

  Experience the excellence and reliability of Veranex Pathology Services, where science and innovation meet to advance preclinical research.